2-mercaptoethanol (also β-mercaptoethanol, BME, 2BME, 2-ME or β-Met) is a actinic admixture accepting the blueprint HOCH2CH2SH. it is acclimated to accomplish protein denaturation by its adeptness to carve the disulfide bonds. In addition, the hydroxyl trapping, it can act as a Pharmaceuticalal antioxidant.
For polyacrylamide gel above-mentioned to the abridgement of the disulfide protein, usually at a assimilation of 5% for the SDS-PAGE sample buffer.It included is broadly acclimated because the hydroxyl accumulation confers solubility in water, and reduces the volatility. it is in some RNA abreast action acclimated RNase absolved during corpuscle lysis to remove.
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